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Description
Aphanomyces root rot caused by Aphanomyces euteiches is one of the most destructive diseases of alfalfa. Resistant cultivars have been developed that exhibit race-specific resistance. Transcript profiling was done to gain a better understanding of the compatible and incompatible interactions. Three-day-old seedlings of the check cultivars WAPH-1 (resistant to race 1 strains) and WAPH-5 ( resistant to race 1 and race 2 strains) were inoculated with MF-1 (race 1) and MER-4 (race 2) and RNA extracted 24 h post-inoculation, with three biological replicates. RNAseq using an illumina HiSeq 2500 to obtain 125 bp paired-end reads was carried out with >10.5 million reads/sample. Differentially expressed genes (DEGs) were identified after sequence trimming and quality control. The incompatible interaction in WAPH-1 and WAPH-5 to MF-1 contained the most unique up-regulated DEGs, 637 and 217, respectively. The incompatible response of WAPH-5 to MF-1 and MER-4 appears to be similar with 258 common up-regulated DEGs and only 59 unique DEGs in response to MER-4. Functional annotation of the DEGs was performed using the Blast2GO tool. The unique DEGs of the incompatible interactions were primarily placed into four categories in the Gene Ontology term Biological Processes: 'defense response,' 'response to stress,' 'organic substance catabolic process,' and 'protein phosphorylation'. These results increase understanding of the A. euteiches and alfalfa molecular interaction for development of cultivars with increased resistant to Aphanomyces root rot.
Publication Date
2023
Recommended Citation
Alexander, Jay; Samac, Deborah A.; and Sathoff, Andrew E., "Functional analysis of differentially expressed genes in alfalfa (Medicago sativa) inoculated with Aphanomyces euteiches race 1 and race 2" (2023). Annual Research Symposium. 53.
https://scholar.dsu.edu/research-symposium/53
